Obtaining Recombinant Ulp1: a Summary

The SUMO protease Ulp1 is an important regulator of the cell cycle in the model system Saccharomyces cerevisiae.  When the cell cycle goes awry in humans, diseased states such as cancer and chromosomal abnormalities can result.  Accordingly, a firmer understanding of how cell cycle regulators like Ulp1 work would further our knowledge of how these diseases come to be, and perhaps enlighten our ability to treat them.  With this in mind, I set out to find out how Ulp1 is targeted to its substrates.  Specifically, I cloned discrete regions of the gene coding for Ulp1 into overexpression plasmids, which I then used to create large amounts of purified, truncated protein for use in in vitro reactions.

[Read more…]

Cornwall summary: Nationalism

The study of Cornish culture, and Celtic studies more generally, often leads to discussions of nationalism. Given issues with North Ireland, Corsica, Soviet Union, Zulu, and Burma, ideas about nationhood are extremely important. Because a nation is partly imagined, beyond what history can prove, does not mean it is imaginary or insignificant. When a group is too big for every member to know every other member, the group is defined by shared beliefs, language, or ethnicity. Newcomers are expected to assimilate or remain outsiders.

[Read more…]

Romantic Misrepresentation of Cornwall

‘There is a tinge of superiority felt by holiday makers and newcomers. Patronizing quaint little towns is a pseudoromantic pixies-and-elves approach. It took me years to throw this ridiculous attitude and get to know Cornwall.” (Winston Graham in Poldark’s Cornwall)

[Read more…]