Girolama Bui and the darned P7 gene

This strain is where things have been getting complicated lately. I’ve accidentally used the wrong set of primers for the first try for sequencing the downstream portion of the P7 gene in the B105 strain and though the sequencing “worked” I had to make sure we sequenced parts of the strain that we wanted specifically. This meant redoing the whole sequencing procedure with the proper primers and crossing fingers for luck! Did it work you wonder? Why yes! Yes it did! It proved the old data that seemed to be questionable about the ending region wrong and even extended the predicted end of the gene! Yay science!