Blog #5: DNA Extraction and Amplification

Hello!

The last few weeks of my summer research have been dedicated to the third component of my project, analysis of bacterial community composition. This is done through T-RFLP, or terminal restriction fragment length polymorphism. In order to do this assay, I first had to extract DNA, and I am still in the process of making sure I can get good amplification so that I know I have enough DNA with which to work. I am leaving campus tomorrow to prepare for a family trip, so I probably won’t be able to do T-RFLP until I resume research in the fall. However, I have still gotten some exciting results despite some setbacks in this part of my project.

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