Thermoregulatory Tracing: Overview

As I stated in my previous blog, my project involves using a retroactive in situ hybridization (ISH) for the rat vesicular glutamate transporter (VGLUT2) to test the efficacy of the gold nanoprobes that are used in the lab for axonal tracing studies.  Ultimately this project is a stepping stone for future ISH studies in neurons, which will hopefully help to elucidate the thermoregulatory pathways we have been studying.

Since the study is fairly technical and will not make much sense to the average reader, it does not make much sense to trace my day-to-day activities in the lab.  Instead, I thought it would be more interesting for everyone if I used these blogs to explain each of the techniques in detail and explain how they fit into the overall scheme.  As a result, my next three blogs will separate and explain the steps that I went through to get to the ultimate goal of performing the ISH.  The themes of the next three blogs will be:

Part 1: DNA synthesis and purification

Part 2: Probe Synthesis

Part 3: in situ Hybridization