QBL Goes to Busch Gardens

A few of us from the Quantitative Biology Lab (QBL) finally went to Busch Gardens last week on July 4th, because we had the day off from lab.  We rode all the major roller coaster, including the new one Verbolten, and did our very best not overheat.  The day resulted in some nice lab bonding, and a discussion of returning later this summer.  I concluded my day off by watching the fireworks and eating ice cream in Colonial Williamsburg with some other friends.

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Continued Perseverance

For the last few weeks I have been growing isolated colonies and using these colonies to run Polymerase Chain reactions (PCR), which were then used to run Amplified Fragment Length polymorphisms (AFLP). These procedures were also done on a bacterial population swabbed from the identical bacterial plate used to create the isolated colonies. The AFLP results from the isolated colonies and the bacterial population were consistent in that each showed the same number of dominant poly-C lengths of our gene of interest,  arsS. Necessary procedures have also been done on the isolated colonies and bacterial population in order to calculate a titer for each sample that PCR and AFLP have been done on. The titer will ultimately be used to calculate a mutation rate for H. pylori. The next few weeks will involve continuing trials of the procedures just described and also preparing reagents that will be needed to clone the poly-C region of H. pylori’s genome and insert it into the bacterium E. coli.

Technology and Business

A couple of weeks ago, I had emailed Ms. Michi Barcelon of the Ayala Foundation’s Technology Business Incubator. The Ayala Corporation is one of the country’s most prominent forces, and the foundation is no different. The Ayala Foundation has initiated and supported a wide array of initiatives that have greatly benefitted the country. As mentioned in their website, The Technology Business Incubator “provides invaluable support to technology start-ups and entrepreneurs” and “aims to identify, develop, and promote social entrepreneurs providing innovative solutions to social problems.” In response to my email, the team had originally set up a meeting with me last tuesday. In an ironic twist of fate, I was unable to get into the city due to a storm and flooding. Refusing to give up, we then took the 21st century approach and arranged a skype video call. The storm was just as persistent and eventually messed with our internet so much that our video call was demoted to a skype voice call to eventually just a regular phone call. Despite the technical difficulties, it was a very good meeting. The team expressed how much they liked the project and forwarded their willingness to support me in anyway they could. They asked if I would be available to meet with their technical team as well as some of the entrepreneurs, and I happily agreed.

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