Continued Perseverance

For the last few weeks I have been growing isolated colonies and using these colonies to run Polymerase Chain reactions (PCR), which were then used to run Amplified Fragment Length polymorphisms (AFLP). These procedures were also done on a bacterial population swabbed from the identical bacterial plate used to create the isolated colonies. The AFLP results from the isolated colonies and the bacterial population were consistent in that each showed the same number of dominant poly-C lengths of our gene of interest,  arsS. Necessary procedures have also been done on the isolated colonies and bacterial population in order to calculate a titer for each sample that PCR and AFLP have been done on. The titer will ultimately be used to calculate a mutation rate for H. pylori. The next few weeks will involve continuing trials of the procedures just described and also preparing reagents that will be needed to clone the poly-C region of H. pylori’s genome and insert it into the bacterium E. coli.