The Hard Work is Paying Off

I made cupcakes again.  I call them Brownie Cupcakes with Chocolate Peanut Butter Frosting.  And they were delicious!  Don’t worry, I’ve attached the recipe: Brownie Cupcakes with Chocolate Peanut Butter Frosting

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A (Late) Start

It seems so strange that I am already so deep into my research on the Bray School. I feel like I have been neglecting this blog, but time has truly been flying! To make up for it, I’m going to do a little 3-part series of blog posts: first, how I set myself up to hunker down in Special Collections at Swem, then a few of the things I found while doing primary source research, and finally an overview of some of the secondary sources I’ve been looking at. Today, I start looking through the microfilms of the Virginia Gazette, and it is my firm goal to write a post the instant I’ve finished them!

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Exciting New Frontier

This previous week of lab work has been exciting because I have moved on to a new portion of my project. I ran PCR on a bacterial population in order to make several copies of my gene of interest (arsS). The PCR product was then used to perform a cloning reaction, which essentially placed the amplified arsS genes into plasmids, which are independent circular strands of DNA. These plasmids were then introduced to E. coli cells in a transformation procedure that caused the E. coli cells to incorporate the plasmids into their cytoplasm. As of now, I am growing these E. coli cells on Agar plates and will pluck the colonies once they have grown large enough. These procedures are exciting because I am one step closer to being able to perform tests for the mutation rate of the poly-C region in E. coli so that I can compare it to the mutation rate of H. pylori.