Continued Diligence

At the same time that I was doing the procedures talked about in the previous blog post, I was also continuing the procedures that lead up to the AFLP protocol. After some initial troubles with the sequencing machine that carries out the protocol, I obtained results that were consistent with results from previous AFLP protocols done. So far, each AFLP has revealed the same number of dominant poly-C lengths within each bacterial colony and population. In the future, we will try to stress the environment of the bacteria to see if the number of dominant poly-C lengths will increase or decrease due to environmental stress.

2 Hybrid Screen

Hello All!

I finally performed my yeast two-hyrbid screen a few days ago!

I crossed my bait, the protein E6-AP, with my prey, the human fetal brain cDNA library in it. I had to prepare a concentrated overnight culture of my bait strain. The next day, the library strain was combined with the bait strain. Then the cells were put in a shaking incubator over night. After 24 hours, I plated my mated culture. Plating the mated culture was an ordeal, because I had to pipette 100 μl of the mated culture on each plate. Mind you, I had plate fifty 150 mm plates. These plates were missing certain amino acids, in order to select for true interactions. The yeast will take 5-8 days to grow. In my last blog post, I will let you know how many colonies grew!

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Quartz and Lords

Monday, July 30th

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