21 Mysteries

Hello all!

Here goes my final blog post for the summer…

In order to select for reporter activity, I identified colonies that were growing on the quadruple selective plates (- TRP-URA-HIS-ADE). After performing the yeast two-hybrid screen, I had a total of 21 blue colonies on all of the plates! Each blue colony represents a protein that interacts with E6-AP. I have been home in California and away from lab, so I have not yet extracted these proteins and then sequenced them in or der to find out what they are. I cannot wait to head back to lab in a few days to discover what these proteins are! Hopefully I will find new protein interactions with E6-AP.

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