Paradox of Research

“Nothing we do in lab applies to what is happening in the wild!” Dr. Heideman tells us the story of his Ph.D. lab PI exclaiming this very unsettling statement. This statement is unsettling not only because it claims to invalidate  what thousands of scientists are doing in labs around the world, but also conveys a stark reminder of the difficulty involved with our attempts to understand the world around us. Similar to the Heisenberg uncertainty principle where location and velocity can not be known at the same time, it seems that scientists are hard pressed when needing to both describe overarching principles of nature with the highly specific and complex mechanisms. This is because the overarching principles of nature are seen by observation of hundreds of variables interacting with each other. One variable defined and influenced by the others. However, to understand the specific mechanism of one variable, one must isolate it and investigate it without the interference of the other variables. But without the other variables acting on this one isolated variable, is this one isolated variable still reflect the variable that is being acted upon in the wild by hundreds of other variables? Because we can only study specific mechanisms by changing the object of investigation drastically from it’s original form, it is hard to know that what is going on in the wild is being reflected in the lab.

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Post #4: Perfusions

One needs to preserve brain tissue and cells in order to see neurons. To do this, we perfuse the mouse. A perfusion runs preservative solution through the mouse in order to keep the integrity of the brain cells as the tissue undergoes sectioning and immunocytochemistry. We run a solution called Zamboni’s fixative through the mouse to preserve tissue.  Although commonly  used for electron microscopy, Zamboni’s fixative is  a good general purpose fixative useful in preserving the brain tissue that we are examining. Zamboni, as we call it, is a phosphate buffered combination of paraformaldehyde and picric acid. It stabilizes cellular proteins without being destroyed by tissue fluids.

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Getting More Help in the Race Against Time!

I know I’ve been silent for almost six weeks, but so much has happened since my last post! For one thing I finally got to interview my aunt and some other interesting characters. But that is another post altogether. What I want to tell you all about now is the week or so leading up to the interviews.

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