Using HPLC-MS to identify proteins from wild type and mutant strains of Heliobactor pylori.

My name is Emily Parrish and I am an incoming senior Chemistry major and Computer Science minor completing a research project in the Chemistry Department.  My project involves the collaboration between Professor Poutsma and Professor Forsyth in the Biology Department.  The goal of our project is to identify which proteins are differentially expressed in several mutant forms of Heliobactor pylori.  Proteins will be extracted from H. pylori samples and separated using gel electrophoresis.  Individual protein bands from the gels will be excised and subjected to enzymatic digestion using trypsin.  The peptides will be analyzed using High Pressure Liquid Chromatography in conjunction with Mass Spectrometry.  By comparing the expressed proteins mutant strains versus the wild type, we hope to further our understanding of gene regulation.

W&M Memory Matters in Spain: Franco’s Repression in Cádiz

My name is Robert Bohnke, and I am a freshman intending to complete a major in Hispanic Studies. I’ll be traveling to Cádiz, Spain through the W&M summer program, and completing an independent research project on historical memory related to the repressive dictatorship of Francisco Franco. I plan to focus my independent research project during my study abroad on the effects of the Ley de la memoria histórica in Cádiz. This national law passed in 2007, mandated a renewed initiative on the part of the government of Spain to support projects that foster historical memory, including the exhumations of the mass graves left behind by Franquismo.  During my time in Cádiz I plan to interview people whose families were affected by the violence of the Franco era and the following pacto de silencio, which survived past Franco’s death in 1975 and discouraged any public display of grievance for lost loved ones.

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