Wrapping Up

In concluding our research, we didn’t see much of a difference in AChE staining between the two hemispheres in the three rat brains from Hampton-Sydney. We are going to try the stain one more time, because it was a bit faint, and just to make sure we’re not missing anything. We will use new tissue sections for the second round of AChE and Parvalbumin staining within the next couple of weeks. With so many steps in each staining procedure, one small miscalculation can throw off results. However, it could just be that the results are accurate, and that there is no difference in AChE staining with Anti-Chat saporin, which would refute our hypothesis for the pilot study.