Data Collection in Chaguite: Part 2

 

The second key component of data collection to answer the research question:  How does the presence of natural resources in a community affect interpersonal relationships for those nearest or farthest from the resource? We managed to reach most of the houses in Chaguite, performing over 30 interviews about the community’s infrastructure and communication networks. Time spent at each household ranged from 11 to 30+ minutes, with some interviewees giving elaborate responses regarding ongoing projects, concerns, or personal experiences. Some respondents were easier to understand than others, and some had more trouble understanding my Spanish than others. There were a few who didn’t really want to “play ball,” aka they didn’t provide responses to the interview questions. I guess I had anticipated a little of that, but it still caught  me by surprise. On another note, by the end of the interviewing, I no longer had to look at the protocol and could recite the entire preamble from memory! That helped the flow of the interview seem more natural and less intimidating to the respondents.

[Read more…]

Data Collection in Chaguite: Part 1

My time in Chaguite was mostly spent conducting interviews at each household. As always, the paths between houses were long and seemed to be uphill in every direction. Along with the MANOS members that I traveled with, I also managed to pull off a meeting with Chaguite’s Water Committee, despite our lack of native Spanish speakers (this is the first trip where we have never had one present). The meeting was brief; we summarized MANOS’s research goals and processes for this trip, asked about any progress with the Engineers Without Borders (EWB) water access project, and left the floor open for any questions or concerns. Direct contact between EWB and community members appears limited, but the attitude towards the project is overwhelmingly positive.

[Read more…]

IF images

IF images

Here are some of the images I obtained during the summer with the confocal microscope. These are HeLa cells exposed to different conditions in order to see how different factors affect localization of CHIP.  The table in the word doc shows different channels collected by different filters of the confocal microscope after excitation of sample with photons. It was really cool to be able to learn so much from this technique. The images were then analyzed via Fiji image processing program. I was able to combine these channels to illustrate merged image. Obtaining images was probably the hardest part and  just optimizing conditions for images. I look forward to learning more about using the microscope in order to help me collect data in the future. After my experiment was over, I gave an oral presentation to two groups at MPI at one of the meetings. It was great to get up there and tell them about my work.  I look forward to sharing my work at W&M soon.

Conclusions

I wanted to post some images of my IF experiment. The computer is not liking that too much so I will just save that for later in September for my poster presentation. Instead here are my conclusion and what was accomplished during this study. THANK YOU. I have learned so much and this opportunity without a doubt will help me in my future career as a biomedical scientist.

[Read more…]

THE END?

Basically the following describes what I did over the summer. In addition to this summary, I learned how to do the entire Western Blot process and got introduced to chIP process. It was an unforgettable summer with so much skill learning. It’s unreal how much I benefited. I know I will carry these invaluable skills with me for the rest of my life especially as I look forward to conducting research in medicine and diseases. COULD NOT BE MORE GRATEFUL for what I have received… I will make it count. Thank you.

[Read more…]