The Good, the Bad, and the Ugly- A Western Blot special

My last post mentioned my successes in TR alpha GFP trapping but unfortunately this good fortune has come to an end. I have been having trouble with TR beta and standard eGFP. So far the IPO7 antibody shows an interaction with TR beta, but a GFP and IPO7 interaction seems to exist, which is not supposed to happen.

I need to focus more on my washes. There are various washes during the GFP trapping procedure and being more careful may help with the accuracy of the results. Also, during the western blot procedure, there are also various washes. I will try to modify the washes just to see if this makes a difference.

Hopefully the next blog post will include some promising results regarding TR beta interaction with IPO7!