Sucesses and failures

The last time I posted, I wrote about my problems with IPO7 and TRB1. I picked apart my procedure and tried to figure out where I was going wrong. After a few tweaks (and crossed fingers) I finally got a clear western blot exposure. The two replicates I have seem to suggest that TRB1 interacts in vitro with IPO7. The GFP trapping that went alongside this trial reinforces the accuracy of the blots.

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The Root of the Problem

This last week of summer research has flown by. I can’t believe it’s all over! After returning from Maine, I was quickly put to work entering all of the data for newly recruited trees into a spreadsheet, which took about two full work days. Not the most glamorous of jobs, for sure, but I’m happy to have gotten it out of the way early.

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Final Week-Best Results

Given some fears about my the properties of my particular azobenzene compound, we ordered a particular reagent to synthesize another azobenzene that will conserve all of the helpful properties of my current one, and allow it to recover faster. This should make my experiment much faster to run and overall more easily-applicable in the future.

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Initial Results with Photo-Acid Generator

I began retesting the remainder of my azobenzene sample to ensure that it has not decomposed over time. NMR and UV/Vis absorbance spectra confirmed that it was still good! With that settled, I could pick up where my project took a break in the Fall and hopefully overcome some of our hurdles,

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Back to Photo-Acid Generation!

As a result of the problems associated with working with my photo-base generator and the lack of options in choosing a new one (photo-base generating compounds are not very heavily studied), I decided to return to photo-acid generators, a type of compounds I worked with last Fall. The most promising was an azobenzene compound that released acid via a photo-isomerization process. It also matched very well with my nanoparticle polymer, PPE, to undergo FRET (Fluorescence Resonance Energy Transfer), the phenomenon that I am studying.

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