Looking forward…

I was really happy with the protein I was able to synthesize, the experiments I was able to conduct and the results I received! By analyzing these results, I have been able to come up with new ideas for future experiments we can conduct by incorporating different UAAs into GFP. Continuing with the 3FY work done this summer, I would like to incorporate other fluortyrosines including di- and tri- fluorotyrosines. I would conduct similar titration experiments to see if the pKa shifts through the addition of fluorines. Finally, I will conduct similar fluorescence spectral analysis on the ortho-nitrobenzyl tyrosine GFP as well.

Thanks for reading!