Characterizing the Drosophila Germline Stem Cell Niche: Future Directions

It’s been about 3 weeks since leaving campus and pausing my research project for now. Reflecting back on my summer of research, I am happy with the progress that’s been made. I helped develop a new process for imaging gonads in vivo using phytagel, found a type of FBS that supports gonad differentiation ex vivo, and optimized the tracking procedure for specific cell types within the gonad using Fiji Track-mate software. There were also come set-backs this summer. It was discovered that the flies used for imaging were contaminated. It was very important that we realized this though so that we can expand a clean line of flies for use in future experiments.

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Evaluating GED Programs

Evaluating GED Programs

Evaluating GED programs can be pretty difficult since every program can operate differently. There are no federal mandates concerning correctional education in states as far as I’ve found. To top it all off, operationalizing the success of a GED program differs for each researcher. Barbara Wade analyzed 13 articles in a literature review called Studies of correctional education programs. She had two goals: (1) to look at evaluation techniques of correctional education programs and (2) to look at the analysis techniques. The two most common evaluation techniques were recidivism and education achievement. Sounds pretty straightforward, right? Wrong. [Read more…]

Almost at the End…Connecting to Authors’ Lives

Hello, again! I thought July went by fast, but August (the Sunday of the year) has been flying by. Here’s what I’ve been up to since the start of the month.

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Inflammation Pathways

My model had no inflammation pathways which I wanted to add as part of the developing features of Alzheimer’s Disease. Therefore, I added three to four reaction pathways that ultimately resulted in the inflammation of the cell. These pathways include the AGE/RAGE pathway which is activated when the AGE  protein and the RAGE protein come together to form a complex. This complex then results in the creation of reactive oxidative species (ROS). The activation of ROS then activates the NFkB pathways which ultimately results in the inflammation of the cell. Unfortunately, the model is currently not accessible to me therefore I can not further explain the reaction pathways in greater detail.

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First Pathway

The first reaction pathway that I added to my model was the JAK/STAT pathway. I was hesitant to add my own pathways to the model for the fear of accidentally deleting previous work or adding an incorrect pathway. However, after overcoming that initial fear I began to model the JAK/STAT pathway. This pathway began with the phosphorylation of the JAK2 protein by activation of the Angiotensin II receptor and Angiotensin II coming together to form a complex. The phosphorylated JAK2 protein then went to on activate the phosphorylation of STAT1 and STAT3 protein which formed a heterodimer, which is a complex of two separate proteins. This heterodimer is then transported to the nucleus. Next, the STAT3 protein and the cJun protein form a complex that activates the transcription, and then translation of the protein thrombin which ultimately assists the body in the coagulation of blood.