A Summer Transformation: the SAC3 gene in S. cerevisiae

Hello, all! The past few weeks have been a bit more promising in the lab. I’ve begun preparations for my gene knock-out, which I will be performing on a previously sequenced gene, SAC3. I worked with this gene in the Spring semester. In order to prepare for my knock-out, I first had to transform the yeast to be able to take in this gene from the environment. Basically, I put the yeast under stress, which then allows for them to take up DNA from the environment.

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June 18-26 Pyruvate Kinase Inhibition

Pyruvate Kinase Inhibition Continued

With the chosen method to determine the activity of pyruvate kinase, there are two stages where time can be varied. Initially, I tested the inhibition of pyruvate kinase after varying the time at both stages. Each molecule must also be tested at multiple concentrations. There were just too many variables of interest.

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Research Progress Update

Hello All!

We are now a few weeks in to the Summer 2018 research session and there are many developments I would like to relate.  After consultation with my lab mentor Dr. Shanta Hinton and lab alumnus Patrick Christian, I have added a computer-driven element to my project.  Using the Broad Institute’s open-source program CellProfiler, I have been able to characterize the effects of our protein of interest, MK-STYX, in thousands of different cells across more than a dozen different parameters.  This has helped further visualize MK-STYX’s impacts on autophagy factors like autophagosomes (cellular garbage trucks) and autolysosomes (cellular recycling centers).  Additionally, I have been utilizing the genetically refined BacMam virus to gain important insights into the inner workings of the cell.  This autophagy sensor lights up various points of interest and allows for them to be compared, counted, and analyzed across different experimental groups.  It has been an excellent time in lab so far, and with the help of Dr. Hinton and my brilliant lab mates, I expect to see many more intriguing research findings this summer.

Update 2 on iOS smart home application analysis

During the past 2 weeks, I have been working on the iPhone 4S that we just purchased. In last week, I learned about different types of jailbreak, tethered, semi-tether, and untethered. And I did a semi-tether jailbreak, which means that I can still use the phone when I reboot. The reason for jailbreaking iPhone is to enable us to use a third party tool, Clutch, to decrypt Apple Store applications so that we can perform further static analysis.

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Partnering with the Nansemond River Preservation Association

This summer I will be analyzing the importance of environmental education in education among minority students in Suffolk Virginia. I have had the great fortune to be able to partner with the NRPA, a local nonprofit it Virginia that monitors water quality in and around Suffolk, Virginia and provides several outreach programs. I will be establishing a supplementary hands-on education program that can be implemented in the summer school enrichment camps in Virginia and then posting them online for the general public to access.