Natural Products Synthesis (2)

The most important part of our research in week 3-4 lies in the successful synthesis of prenylated methyl-p-benzoquinone products with pure NMR spectra obtained for publication. This involved prolonged reaction monitoring, doing overnight NMR experiment, and a little bit of luck. Luckily our experimental data confirmed our research hypothesis and the synthesized products are on their way to a lab in Germany for further biological testing!

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Protein Immobilization Research Weeks 5-6

Now that I feel comfortable in the lab and with my project, the past few weeks have focused on problem-solving and optimizing reactions. While I was able to express one of the SSO mutants using plasmids created by the lab’s previous graduate student, I was not able to successfully express the other two mutants or the wild type. Thus, I’ve spent the last few weeks cloning the SSO mutants and wild type into a functional plasmid that will hopefully allow us to express. I’m anticipating positive results later this week and into next, which will allow us to take further steps in the immobilization of this protein.

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