Vibrio Fischeri Luminescence

My project is primarily focused on whether the adaptive radiation of various strains of V. fischeri from various ecological niches affects response to physiological stress. So far, I’ve been growing smooth morph and wrinkly spreader strains in various concentrations of sodium chloride. Growth has been evaluated using a spectrophotometer to estimate cell density in experimental cultures.

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End of an Era

Today is my last day lab, and this last week has been packed. We finished up several trials, one of which we’ll actually be able to score for data since the cells look amazing. We also figured out the concentration of retinoic acid that we want to use for N2As since we were having some issues with actually seeing neurites and cell differentiation. When we fixed the cells, we didn’t see neurites and the cells looking very unhealthy. We realized that this was likely due to serum starving the cells the entire time we stimulated them (which was what was suggested to us by the provider of the new cell line) since we had been adding the RA to RPMI medium with 0.5% FBS instead of the normal 5% FBS, 10% HS. We made up a sample six-well plate of different concentrations of RA with normal RPMI after serum starving the cells overnight, and after 24 hours we were already starting to see neurites in each of the wells and the cells looked healthy. We found that the concentration of RA we had already been using was most effective, we just needed to stop serum starving for such a long period of time. Our future trials of N2As are sure to look much better.

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Analysis of Milkweed Genotyping

With just a few individuals left to send off as redos, most of the focus on the milkweed project has moved towards analysis. Analysis is inherently tricky because there is a lot of trial and error. There are essentially a few options for analysis. You can use someone else’s software, you can modify someone else’s to fit your needs, OR you can make your own. The last one is obviously extremely time intensive, so our hope was to find a software we could use to fit our needs.

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Mentors are Necessary: The Need for Mentors to Underserved Children

Hi everyone!

It’s been a busy couple of weeks in the lab but I am back and have lots to talk about. Last time I had some pretty exciting results to share with y’all and next week I’ll have some more findings to talk about. This week I wanted to take a quick step back from my data and discuss the necessity of mentoring as a whole.

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