Protein Immobilization Research Final Update

As the last week of research approaches, it is rewarding to look back and see all that I have learned. The last two weeks have been extremely productive: I’ve successfully cloned SSO into another plasmid and expressed both the wild type and a mutant version. Furthermore, I’ve been able to run reactions showing the sustained activity of the mutated protein, and thus its potential in biosensors. My aim now is to express at least one more active mutant, and immobilize the mutants in different conditions. If research continues to progress the way it has, we will hopefully have enough for a paper in the fall.

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Nighttime vs Daytime Toad Movement

This field season has been very busy with tracking toads! I have been following two species of toads, American and Fowler’s toads, at three sites and have had great success. The main setbacks I have faced has been predation and being unable to locate the tagged toads. We had one toad definitely eaten by a snake, confirmed with a strong signal coming from the inside of the snake, as well as a few others that we suspect met the same fate. Even when they are not eaten, sometimes I am unable to relocate the toads. Despite this, I have been able to track many toads this summer and I am getting great data!

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