Workable Data!

After literal months of processing and tweaking, I finally have usable data. This is extremely exciting for me as this project is moving into the next stage. Right now I’m currently working on data cleaning. As a lot of the data was low coverage, these reads needed to be removed. I’ve written several simple processing scripts in both Python and R to trim data and separate it into different data frames that I’ll begin analyzing separately. I’ve begun very rudimentary analysis in R on one of my species of Mimulus, naiandinus, and have found some very preliminary results (displayed in the attached boxplot). The aim of this research is to estimate the diversity of populations. In this case, diversity is represented by the number of nucleotide differences divided by the total number of nucleotide overlap in any given gene. In this way, we can use the number of base pair differences as a metric of genetic diversity.

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End of the summer

I can’t believe that the summer research has come to an end! So far, I have successfully synthesized and identified our products of interest in the lab, and they were sent for biological testing. Byproducts were also identified, which provides further insights for the reaction mechanisms. The new reaction has worked out well, with some preliminary yet promising results. The next step would be further purification of the products to confirm our hypothesis. If everything works out well in the end, it will be a first-time synthetic route for a natural molecule that has its biological activities confirmed in literature.

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Update

In researching how new media affect the public and private spheres in relation to democratic theory, it was important to have a grasp on some of the most influential formulations of the ideal public sphere from the past. One of the prominent theorists of the ideal public sphere was Immanuel Kant.

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