Week 3

Optimization trials have been started. As of right now we are trying to optimize transfection and stimulation. We have seeded cells into wells to try and see the proper amount of time to allow the plasmids to transfect into the cells. We are also trying to optimize the amount of retinoic acid to put on cells to allow them to differentiate without dying or getting overly stressed. The results of these experiments will help will my overall experiment.

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