6th Week of Summer Research

Hi Readers,

In our cellular and developmental biology lab, we utilize different fixations to visualize the chromatin and proteins of interest. The two major fixations we utilize are an overnight methanol and 10 – 15 minute paraformaldehyde (aldehyde). Methanol fixations precipitate the proteins and preserve the structure of the proteins, but can cause denaturing of small soluble molecules. Paraformaldehyde cross links proteins by free amine groups, which ultimately forms intermolecular bridges (1).

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Week 3

I got down to work my third week here in the Philippines. 

 

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Week 7: New addition and dehydration reactions

This week the focus was on trying addition reactions in order to move the current product closer to one of our goal molecules. The first two reactions were run next to each other. One was a grignard addition using isopropyl magnesium chloride and then other was an organolithium reaction using n-butylithium. The grignard, which had been run once before on a smaller scale, was run using ether as the solvent instead of dichloromethane. This reaction did not actually occur on the larger scale and we think it is because the solvent could not get the starting material completely in solution. However, all the starting material was recollected so nothing was lost from this trial.

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Getting Good Bands

MVIMG_20190722_142925_2

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Art Outside of the Getty

I can’t believe summer is over halfway over and my time here is dwindling! The summer has been flying by. Nevertheless, I’m determined to squeeze every last bit I can out of this season while it lasts.

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