Week 4

During this week, I attempted to optimize transfection. One of our plasmids did not seem to show under fluorescent microscopy, which meant either the plasmid was in the cell and wasn’t showing fluorescence, or the plasmid wasn’t even in the cell to begin with. Because the presence of fluorescence is how we detect the presence of a protein, we needed to make sure transfection was working properly. I attempted to try different amounts of the chemicals we use to transfect DNA into our cells to see which one had the best results. Transfection can be a toxic process for cells, so it was likely that we were using too much chemical or DNA.

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