Week 5

Optimization has gone semi-well. We have seemed to reach a conclusion about RA concentration with 20 micromolar concentration being the most ideal. We have also realized that a major issue with transfection was due to the fact that we did not wait for the cells to become completely confluent. Confluency is when the cells are adhered to the bottom of the flask and are growing happily. If we do not wait for the cells to become confluent, and we have them undergo a toxic process like transfection, it makes sense if they do not grow to be happy. We have decided to add another day to the procedure.

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