Week 6

Canada! We have officially arrived at Mount Sinai Hospital to continue our project. We have started N2A cells to continue optimization with the help of a PhD student who has experience with N2A cells. We have decided to test whether poly-D-lysine helps with adherence of the cells or not. It does look like it is necessary to keep the N2A cells from flying off. We have also learned lab techniques like cloning, which is when you insert a gene of interest into a vector with restriction enzymes, an extremely useful skill for us in biochemistry/molecular biology labs. We have also seen the mass spectrometry room and their 8 mass spectrometry machines which is CRAZY, considering each one costed a fortune plus upkeep money on top of it all.

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