Final Thoughts

Hi everyone,

I realize that I’m very late at posting this and I apologize for that. I hope everyone is faring well at getting adjusted to the semester after a windy start.

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New directions

Hi guys,

In one of the first posts describing the mutant, I mentioned that rrf-3 had spermatids with off-center chromatin and a quantification of this defect is one of the new pursuits in the whole rrf-3 project. To quickly recap, the rrf-3 gene product is involved in an RNAi pathway in C.elegans spermatogenesis, turning genes on and off (essential in development) by destroying their message (RNA) or preventing its translation to protein.

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Major Sperm Protein: life at the leading edge

Hi!

The main goal of an activated spermatid is to get in touch with an oocyte and deliver a haploid nucleus to it (fertilization). How this fertilization is done is beyond the scope of our lab’s work. The diagram below (L’Hernault, 2009) outlines well what we’re focusing on, the steps prior to fertilization.

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Fluorescence Microscopy and all the pretty colors

Hi guys,

So far I’ve been posting about the characterization of the rrf-3 mutant and I mentioned that fluorescence microscopy was used in analyzing cellular structures in comparison to the wildtype phenotype. We can better appreciate what the pictures reveal if we understand the basics of this amazing technique, so here’s an attempt at it.

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Kiss meiosis; when good sperm goes bad

Hi guys,

Over the past three weeks, we’ve been making fairly good progress in Dr. Shakes’ lab. I have been brushing up on some of the techniques and protocols used in cellular biology, I’ve been growing up a healthy strain of worms, and have been reading some of the scientific literature related to my topic.

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