At the End of the Summer

And here it is. The moment we have all been traveling towards: the end of summer research! I am actually rather sad that the summer is pretty much over already. It has been a festive summer with some great results!

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The Final Countdown

As I enter my final week of research, I am almost rushing to tie up a couple of projects so that they do not dangle the two weeks before I come back to school.

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Dear Diary: A Victory!

Dear Summer Blog Post,

When we last talked I complained about the woes of having bad competent cells with which to make protein(unsuccessfully). That problem has been rectified and science and results can continue. And so they do!

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Check your cells before you wreck your cells

Amazing that six weeks have already passed! While research has been continuing at a rapid pace, the results, unfortunately, have not. Last time I mentioned that we haven’t found the right conditions to synthesize the proteins that we want to study. After much repetition of experiments and confused looks at the results (for instance, the protein that we work with is called Green Fluorescent Protein…it was not green) we have finally (hopefully) found the source of the hold-up: bad cells. That is right. The cells that we used to synthesize the protein that we then study and manipulate had somehow become contaminated such that the cells were not properly taking up the plasmid (DNA material) that they needed to obtain the ability to manufacture the protein of interest. New stock cells have been made and research can continue with more promising results.

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Ready, Set, Synthesize Protein!

So far this summer I have participated in Dress-Up Mondays (this Monday was rather formal!), liquid nitrogen ice cream, lab meetings, lab dates and excursions to lunch and ice cream (again because we just cannot get enough), karaoke in lab as we work, and other shenanigans.  But this blog is supposed to be on the research that I am doing? Very well, I can discuss that too!

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