A look back at summer

I cannot believe my final summer at the College of William and Mary came to a close about a week ago. It feels like just yesterday I was entering Allison lab as an eager sophomore striving to learn more about the field of research. It has been a rewarding experience to say the least.

[Read more…]

The continuing battle with the Western Blot

I have been continuing my battle with the western blot. I have had blots expose perfectly as well as blots come out of the machine completely blank. These last few weeks have taught me to be extra vigilant in my procedural work as one small mistake can ruin 4 days worth of work. My most recent blot was quite pretty but the level of trapped GFP was too low. This can be attributed to a low transfection rate. I have spent the last week tweaking the transection assay to ensure that I can get the highest transfection rate. It makes sense because the more protein you have, the brighter your blots will expose, which allows for easy analysis.

[Read more…]

Sucesses and failures

The last time I posted, I wrote about my problems with IPO7 and TRB1. I picked apart my procedure and tried to figure out where I was going wrong. After a few tweaks (and crossed fingers) I finally got a clear western blot exposure. The two replicates I have seem to suggest that TRB1 interacts in vitro with IPO7. The GFP trapping that went alongside this trial reinforces the accuracy of the blots.

[Read more…]

The Good, the Bad, and the Ugly- A Western Blot special

My last post mentioned my successes in TR alpha GFP trapping but unfortunately this good fortune has come to an end. I have been having trouble with TR beta and standard eGFP. So far the IPO7 antibody shows an interaction with TR beta, but a GFP and IPO7 interaction seems to exist, which is not supposed to happen.

[Read more…]

GFP Trap, SDS Page, and Western Blots

Hello,

It has been a month since I started my summer research. I took a brief vacation this past week but I am back and ready to resume my work in the lab.

[Read more…]