Week 1– molecular Reagent Preparation

As I mentioned in the abstract, the goal of my research is to investigate mitochondrial features and its relationship with neural progenitor cells (NPCs). Therefore, it is necessary to construct certain plasmids (i.e. molecular reagents) to mark both the NPCs and mitochondria. Thus, constructing a vector plasmid requires three donor plasmids (see below): 1. a promoter region (5′ entry plasmid); 2. a sequence that is responsible for marking NPCs (middle’ entry plasmid); 3. a sequence that is responsible for marking mitochondria (3′ entry plasid). For this week, I put a sequence that generates red florescent protein into a 3′ entry plasmid as a donor plasmid.

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Welcome to Winterthur

About me:

Although I grew up in the Delaware-Pennsylvania area, this summer is the first time I ever visited Winterthur Museum, Gardens, and Library. For the next ten weeks, I will be tucked away at a desk on the sixth floor in a room full of quirky decorative curiosities, learning from Winterthur’s curatorial department. I am Winterthur’s first Woody intern from William and Mary, and am very lucky that the Woody Internship in Museum Studies makes it possible for me to experience behind the scenes museum work this summer.

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Columns, Columns, Columns!!

During the second week of research, I realized what it meant to work in a synthesis lab. The realization came with my first column.

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Each year the Roy R. Charles Center provides research funding in a variety of disciplines for William and Mary undergraduates. This summer 57 students will devote 7 weeks to a project and blog about their experience. Follow their progress, ask questions, and get to know what exciting research W&M students are conducting!