Here are some of the images I obtained during the summer with the confocal microscope. These are HeLa cells exposed to different conditions in order to see how different factors affect localization of CHIP. The table in the word doc shows different channels collected by different filters of the confocal microscope after excitation of sample with photons. It was really cool to be able to learn so much from this technique. The images were then analyzed via Fiji image processing program. I was able to combine these channels to illustrate merged image. Obtaining images was probably the hardest part and just optimizing conditions for images. I look forward to learning more about using the microscope in order to help me collect data in the future. After my experiment was over, I gave an oral presentation to two groups at MPI at one of the meetings. It was great to get up there and tell them about my work. I look forward to sharing my work at W&M soon.
I wanted to post some images of my IF experiment. The computer is not liking that too much so I will just save that for later in September for my poster presentation. Instead here are my conclusion and what was accomplished during this study. THANK YOU. I have learned so much and this opportunity without a doubt will help me in my future career as a biomedical scientist.
Basically the following describes what I did over the summer. In addition to this summary, I learned how to do the entire Western Blot process and got introduced to chIP process. It was an unforgettable summer with so much skill learning. It’s unreal how much I benefited. I know I will carry these invaluable skills with me for the rest of my life especially as I look forward to conducting research in medicine and diseases. COULD NOT BE MORE GRATEFUL for what I have received… I will make it count. Thank you.
I write to you from my station at the NAACP National Convention in Las Vegas, Nevada. First and foremost I would like to say that hell hath no fury like the Vegas heat. It is extremely hot here at all times. But besides that I am enjoying every minute of the convention and of my internship.