In researching how new media affect the public and private spheres in relation to democratic theory, it was important to have a grasp on some of the most influential formulations of the ideal public sphere from the past. One of the prominent theorists of the ideal public sphere was Immanuel Kant.

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End of Summer

This last week has been surprisingly busy. There were a lot of loose ends to tie up before we left for 3 weeks. We finished another round of plasmid preparation so we have enough supplies to last us through the Fall semester. Plasmid preparation can be tedious so getting a head start on that will make our lives easier in the coming months. We also had to freeze down our cell lines. Since we won’t be here for the next weeks, we need to ensure our cell lines will be taken care of. Instead of replacing medium and flasks, we will freeze the cells down in nitrogen to keep them viable for experiments. We have also taken care of various lab maintenance issues like ordering more equipment to restock the lab. I also took the time to score a previous successful trial. Results were interesting, and I definitely was surprised at how much time it took me. Overall it has been a relatively smooth week in lab. I look forward to continuing my research in the Fall.

SLX5 and Huntington’s Disease: Second Update

In my last blog post, I spoke about developing a protocol to verify findings from a new data set. This has been my focus since that update.

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Mentors are Necessary: The Need for Mentors to Underserved Children

Hi everyone!

It’s been a busy couple of weeks in the lab but I am back and have lots to talk about. Last time I had some pretty exciting results to share with y’all and next week I’ll have some more findings to talk about. This week I wanted to take a quick step back from my data and discuss the necessity of mentoring as a whole.

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Getting Started with Sequencing Analysis

It has been a few weeks and I thought that I would give some updates on my progress so far. I’m currently replicating methods from the paper Sex Speeds adaptation by altering the dynamics of molecular evolution (1). This paper provides a useful computational workflow for identifying mutations from sequence data. This past month I have come across a huge learning curve, using software that I have never come across before, specifically GATK (Gene Analysis Toolkit). My first research task is sequencing analysis, and given that I have very minimal experience using sequencing software, it has been quite a challenge. Nonetheless, I have enjoyed learning a lot from this experience. From deciphering and creating pipelines, troubleshooting errors and running 50+ hours commands (we have a huge dataset), I’m getting closer to having a working dataset that I will use for analysis.

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