Trying not to wing it! – Week 7 Update

Oopsie, I forgot to post this week 7 post when I should have a month ago but here she is:

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Better Luck Nest Time! – Week 6 Update

Like last, this week was not eggstra special in any big way. We had egg dissections to do most days and did some more runs from frozen samples at 4 and 6 days old – nothing new here. The egg dissections were of an older stage (11 days) so we had to figure out exactly how we wanted to extract and dissect those embryos but this was a pretty simple decision and we already have five or six samples in the freezer! We probably won’t use these samples until the fall but its always nice to have stuff frozen and ready to use, and to take advantage of this period of steady control egg production while we can.

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A Pretty Reggular Week! – Week 5 Update

Looking back, it seems like this week was pretty smooth sailing, however it did start with a bump. We have been successfully extracting RNA from embryos that are 4 and 6 days old for a really long time (months in fact). Last week, we tried changing to a younger age and it didn’t work (as somewhat expected). As we wait for the eggs we have to get a little older, we decided to do a few more of the extractions we are comfortable with, to boost our confidence after an unsuccessful week. Unfortunately this didn’t happen.

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We‘re Not Yolking Around! – Week 4 Update

Okie dokie. Well, this week we decided to go out on a limb to see what direction to take our project. You may (probably not) remember that I ended my post last week with the idea that we are looking to incorporate another age of embryo into our project, either much younger or much older, in order to have a better chance of getting interesting and more exciting results from our RNA extraction. Dissecting older embryos is probably the more reliable/easier option since we would not have to change our protocol all too much…but what fun is choosing the easier route? Thus, this week we embarked on attempting to extract RNA from younger, almost non-existent, embryos.

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Nothing to (formalde)Hyde! – Week 3 Update

After three long weeks, this week we finally had some fresh eggs to dissect. My lab partner and I have been doing dissections for a few months so we are pretty good at clean, fast dissections and hadn’t gotten too rusty in our time away. We added the embryos we dissected this week to the freezer where they can sit until we are ready to do RNA extraction from them.

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