7th Week of Research

Hi Readers,

During my 7th week of research, I began working with a mutant strain of C. elegans that does not express the anaphase promoting complex. The APC mutant emb-27 will arrest cells in metaphase I of meiosis, but other cellular processes will continue to occur after anaphase I should be occurring; for example, the residual body still forms when spermatids should be budding from the residual body. This mutant will allow us to continue to characterize SUMO’s role throughout the meiotic division zone, even when the chromatin is not moving through the sequential cellular divisions. I have had some hiccups with the permeabilization step required to allow the SUMO antibody access to SUMO, which is within the cells of the gonad. I will try varying amounts of permeabilization in my next prep to determine the sufficient amount to allow visualization of SUMO.

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6th Week of Summer Research

Hi Readers,

In our cellular and developmental biology lab, we utilize different fixations to visualize the chromatin and proteins of interest. The two major fixations we utilize are an overnight methanol and 10 – 15 minute paraformaldehyde (aldehyde). Methanol fixations precipitate the proteins and preserve the structure of the proteins, but can cause denaturing of small soluble molecules. Paraformaldehyde cross links proteins by free amine groups, which ultimately forms intermolecular bridges (1).

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