Last post for the summer

So, there has been a few problems within the enumeration process of the viral particles.

That is to say, I have not YET been able to count them due to some mysterious interactions between vrial particles, heat, DNase, and backgorund flourescence.

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Do you want the good news or the bad news?

Let’s start with the bad news.

Bad news-

An intergral part of the microcosms is the process of enumerating the amount of viruses in a sample. This process of enumeration is completed via the use of epiflourescence microscopy. Pictures are taken using a program and then the pictures are counted for virus-like particles. Here comes the bad news, the program which is used to take and save the pictures of the samples is not working properly for me. sadface. The program is actually not saving my pictures which makes enumeration impossible. You can not count something when it does not exist, or in this case save. One possible solution would be to take the pictures and immediately enumerate them. Another possible solution would be to label the pictures in a specific way so they do not cause problems with saving with this program. The correct solution is to be determined in the future.

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Here we go again

So T4 decided to be a passive agressive bacteriophage last week.

The microcosms neglected to show any significant flourescence after 12 hours.

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Some minor setbacks

Hello passengers, this is your captain speaking. We are experiencing some  minor turbulence, please fasten your seatbelts and remain seated.  All attempts at being funny aside, the project assessing the duration of a certain bacteriophage’s phase of infectivity is experiencing some tiny (but annoying) setbacks. First of all, let me bring you up to speed.

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Yay phage!


April 26,2010

This summer is going to be exciting!
My project is titled, “Inquiry into the Duration of Infectivity of Bacteriophage in Soil”. (If the title does not get you pumped for this blog, you might need to check your pulse but I digress. )
So what does this mean?
Well, this summer I will being attempting to find out if soil phage actually survive longer and how (tentative on the how). Since soil phage do not have any known way to actively persue their hosts, they should have longer infectivity phases. A longer infectivity phase would allow the soil phage a greater opportunity to actually find a host and lyse it.
To determine whether a soil phage’s period of infectivity is longer than that of its aquatic counterpart, I would create microcosms. The microcosms would allow me to determine the infectivity of a phage virus in a controlled manner and over a period of time.
There are more details to come.
Stay tuned to hear about this exciting experiment!