Final Summary

This summer went by fast! I feel I have accomplished a lot!

The goal of my project was to determine if genes required for sexual reproduction are undergoing selection to facilitate speciation in the yeast Saccharomyces paradoxus. Saccharomyces paradoxus is woodland yeast found in Europe and North America. In a previous lab, my advisor found evidence of speciation in this yeast. In a recent migration event, a European Saccharomyces paradoxus population migrated to North America. This Migrant population exhibited mating discrimination towards potential North American mates while its ancestral European populations did not. This behavior likely evolved in the Migrant population to avoid reproducing with genetically incompatible North American potential mates, as the offspring of inter-population pairings were substantially less viable. This served as evidence for speciation.

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Last Week: Dissection and Replica Plating

This past week, I’ve been performing dissections with the yeast I have successfully transformed. Before the yeast can be dissected, they must undergo sporulation, forming four haploid spores. In a dissection, I separate the spores, placing them in columns on a YPD plate. The picture below is from one of the dissections I performed.

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