A Summer Transformation: the SAC3 gene in S. cerevisiae

Hello, all! The past few weeks have been a bit more promising in the lab. I’ve begun preparations for my gene knock-out, which I will be performing on a previously sequenced gene, SAC3. I worked with this gene in the Spring semester. In order to prepare for my knock-out, I first had to transform the yeast to be able to take in this gene from the environment. Basically, I put the yeast under stress, which then allows for them to take up DNA from the environment.

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