Repotting, Re-PCRing, and the War for the Greenhouse

Life has been hectic since returning to campus for summer research. I dove back into work with a massive repotting of my milkweed. My project requires two stems of milkweed in every pot—on one stem I will simulate insect herbivory, and I will measure cardenolide levels in both to determine whether chemical defense signals are shared through root systems. Splitting and repotting milkweed is an all-day event, mostly because by the end of it you’ll be too dirty and exhausted to want to do anything else. Milkweed naturally occurs in fields and prairies, where plants send out sprawling root networks that take up water and nutrients and anchor the plants so they can grow as tall as over two meters. Though our plants in the greenhouse never grow that tall, their roots still try, and milkweed pots are crammed with tangled and gnarled knots of them. My labmates pitched in to help me out on repotting day, which I will always be thankful for, considering what a huge and messy task it was! We shook out gallons of loose dirt and chisel away more from the roots, so we could trace roots to see which stems are closely collected and can become a pair in its own pot. We do all this while trying not to snap the delicate stems, which grow to about two to three feet in the ISC. By the end of the day, we were all covered head to toe in potting soil, as were the floors of the greenhouse, which we bleached and scrubbed.

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