June 7-14 Pyruvate Kinase Inhibition

Use of Molecules to Inhibit Pyruvate Kinase

With a method to measure the activity of pyruvate kinase, the research could begin. Next, the goal was to investigate the effects of various molecules on the activity of pyruvate kinase. This had to be done in three stages. In the first stage, the molecule modifies pyruvate kinase. In the second stage pyruvate kinase is allowed to react. Finally, in the third stage, a simple reaction occurs to make the product from stage 2 measurable.

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May 30- June 6 Establishing Controls

Stability of Relevant Molecules

For the first 2 weeks of summer research, the goal has been to lay the groundwork for the rest of the summer. The amount of time certain molecules can sit out on the lab top at room temperature before they degrade and become unusable had to be established. Then the effect of freezing and thawing an enzyme multiple times on its activity level was investigated. These things had to happen before the real research could begin. This took several days of control experiments.

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