Closing Thoughts on My Summer Research Project

Hello There!

With my summer projects complete, and with an adequate time for reflection about my results and the future directions of my projects afforded, I now feel ready to complete my last post of the season.  From the data gathered from the autophagosome, lysosome, autolysosome, and L3CB experiments, I have seen first hand some of the fascinating cellular effects that MK-STYX exerts.  With this protein established as an important autophagy player in our in vitro studies, exciting new experimental directions beckon in other organismal models.  In the future, I will explore how autophagy transcription factors, the activators of specific genes in DNA, are influenced by MK-STYX.  Following this, I will investigate the known role MK-STYX plays in clearing misfolded proteins, or protein aggregates, from cells.  I believe this represents an important experimental direction as these variousprotein aggregate types are major factors in devastating diseases like Huntington’s Disease, ALS, and Alzheimer’s.

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Research Final Summary

Final Summary

In the end, we investigated five molecules for inhibition of Pyruvate Kinase. Of the five, two definitively inhibit PK. One definitively does not inhibit PK. Finally, two of them might inhibit PK and need to be tested further.

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