Week 7 – Babalik ako sa “bahay” ko

Hello readers,

 

This week I continued with my two projects: isolating Paramecium and Acanthamoeba. Thankfully, after several days of trying to get the paramecium to multiply in a test tube, it worked! I started with one paramecium that I got from water in the laguna inside the University of the Philippines, and I was able to get five to six parameciums at the end! I was unable to complete the final step of the project as I was out of time; however, I would have done a DNA extraction/PCR in order to determine if the microbe was in fact a paramecium. I was also able to isolate Acanthamoeba from the air conditioning filters. I found an isolated cyst (dormant state of Acanthamoeba) within the gel and I flame-sterilized a scalpel to cut the cyst out and place it onto another plate with E. coli. After letting it incubate for a day, I checked the plates and I found that there was an Acanthamoeba present within the plate! I also helped the salmonella team perform their Vitek operations during my final week. 

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Week 6 – Malapit na yung huling el bimbo ko

Just as Joy and Ethan in the movie Hello Love Goodbye turned a watch that was able to “stop time”, I too wish for a way to stop time to remain in the Philippines for just a bit longer. I can’t begin to express my joy and gratitude for being able to partake in an internship in such a beautiful country I call home. My 6th week in the Philippines was filled with more fun activities and more important experiences within my lab!

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Week 5

During the fifth week of my internship, I worked with the salmonella team once again. I subcultured salmonella strains with Ate Jonah and Ate Issa. I worked in the Institute of Biology with Ate Issa and Ate Jonah and utilized the equipment, Vitek. Vitek is used for high-throughput antimicrobial susceptibility testing (AST). We were able to get 5 isolates from glycerol stocks of salmonella. We kept the salmonella in low temps and glycerol. The low temperature slows down the metabolism of the cells, allowing them to survive for a longer period of time. The glycerol is added in order to prevent the cells from actually freezing due to the low temperatures. For the purpose of the existing salmonella project, Vitek was used for AST, pero it can also be used in identifying unknown bacteria and yeast. Once these unknown bacteria are identified, we can start to create a DNA library. Although the Philippines lacks this specific library, a genomics project is attempting to start building one!

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Week 4

“As I’m getting ready to leave Singapore for the second time I am more at ease with leaving, life and the impermanence of everything…almost as if the universe felt my unease, it allowed me one more day to find peace on the island and just breathe” – Maggie Chu

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Week 3

I got down to work my third week here in the Philippines. 

 

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